Neutrophil Changes in Elderly Mice

This a study based on the increase in neutrophils in secondary lymphoid organs and BM of elderly mice. The first line defense against foreign agent and the innate immune system’s key elements are the polymorphonuclear neutrophils. It has been hypothesized in this study that the occurrence of the chronic low-grade inflammation during inflammaging results in neutrophil functional changes in the secondary lymphoid organs through an autocrine feedback loop. There has been increase in neutrophils in the healthy elderly mice’s lymphoid organs. It has been found that aged and hypersegmented neutrophils have had their presence in the lymphoid organs which are healthy and elderly. Splenic macrophage that comes from the elderly and healthy mice has been found to be exhibiting a decrease in phagocytosis. In the elderly and healthy mice, there has been infiltration of splenic white pulp zones by neutrophils. Female C57BL/6J and Balb/c mice, young, in the age group of 2-3 months, Balb/c, 17-18 months, or C57BL/6J, 22-24 months were obtainable Animal Resources Centre and maintenance is done under the specific conditions that are pathogen free with 3-5 cage/mice on corn-cob bedding at the animal facilities of University of Western Australia. Concentrations of MCP-1, IL-12, IFNc, IL-10, IL-6, and TNFa in plasma samples have been subject to measurement with the use of BD Biosciences according to the instructions of the manufacturer. The calculation of statistical significance is done with the use of GraphPad PRISM 6 (California, USA). The Mann–Whitney U-test and t-test are used in determining the population differences. This study has shown that increase in neutrophils in lymphoid organs of elderly and healthy female mice and infiltrate B- and T- cell lymphoid areas takes place where the pathological disease is absent.